Approaches for targeted proteomics and its potential applications in neuroscience.
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Date
2015-09
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Abstract
An extensive guide on practicable and significant quantitative proteomic approaches in neuroscience research is
important not only because of the existing overwhelming limitations but also for gaining valuable understanding into
brain function and deciphering proteomics from the workbench to the bedside. Early methodologies to understand the
functioning of biological systems are now improving with high-throughput technologies, which allow analysis of
various samples concurrently, or of thousand of analytes in a particular sample. Quantitative proteomic approaches
include both gel-based and non-gel-based methods that can be further divided into different labelling approaches. This
review will emphasize the role of existing technologies, their advantages and disadvantages, as well as their
applications in neuroscience. This review will also discuss advanced approaches for targeted proteomics using
isotope-coded affinity tag (ICAT) coupled with laser capture microdissection (LCM) followed by liquid chromatography
tandem mass spectrometric (LC-MS/MS) analysis. This technology can further be extended to single cell
proteomics in other areas of biological sciences and can be combined with other ‘omics’ approaches to reveal the
mechanism of a cellular alterations. This approach may lead to further investigation in basic biology, disease analysis
and surveillance, as well as drug discovery. Although numerous challenges still exist, we are confident that this
approach will increase the understanding of pathological mechanisms involved in neuroendocrinology, neuropsychiatric
and neurodegenerative disorders by delivering protein biomarker signatures for brain dysfunction.
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Keywords
Brain, differential proteomics, ICAT, LCM, neuron, tandem mass spectrometry
Citation
Sethi Sumit, Chourasia Dipti, Parhar Ishwar S. Approaches for targeted proteomics and its potential applications in neuroscience. Journal of Biosciences. 2015 Sept; 40(3): 607-627.