Role of leptin G-2548A polymorphism in age- and gender-specific development of obesity.
Loading...
Date
2015-09
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
Leptin is involved in the regulation of food intake and energy expenditure, and therefore, is central to adipositysensing
pathway. We examined the relationship of the leptin G-2548A polymorphism with obesity and obesityrelated
anthropometric and metabolic parameters in a total of 394 (239 obese and 155 non-obese) subjects
between 5 and 45 years of age. Body weight, height, waist circumference (WC), hip circumference (HC) and
blood pressure (BP) were measured. Body mass index (BMI) and waist-to-hip ratio (WHR) were calculated.
Levels of fasting blood glucose (FBG), insulin, leptin and leptin receptor were determined, and homeostasis
model assessment of insulin resistance (HOMA-IR) was calculated. Genotyping was carried out by polymerase
chain reaction-restriction fragment length polymorphism (PCR-RFLP). The LEP G-2548A polymorphism showed
association with obesity in children and adolescents ( 18 years of age) but not in adults. However, analysis by
gender stratification revealed association with obesity in girls only. In addition, G-2548A polymorphism showed
association with BMI, WC, HC, fasting blood glucose and serum leptin levels. This suggests that G-2548A
polymorphism may influence the susceptibility to metabolic disturbances and obesity at an early life. Further
investigation with a larger sample size is required to validate the effect of LEP G-2548A polymorphism in obese
Pakistani girls.
Description
Keywords
BMI, LEP G-2548A, obesity, Pakistan, polymorphism
Citation
Shahid Adeela, Rana Sobia, Mahmood Saqib, Saeed Shahid. Role of leptin G-2548A polymorphism in age- and gender-specific development of obesity. Journal of Biosciences. 2015 Sept; 40(3): 521-530.