Riboflavin transporter-2 (rft-2) of Caenorhabditis elegans: Adaptive and developmental regulation.
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Date
2015-06
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Abstract
Riboflavin transporters (rft-1 and rft-2), orthologous to human riboflavin transporter-3 (hRVFT-3), are
identified and characterized in Caenorhabditis elegans. However, studies pertaining to functional contribution
of rft-2 in maintaining body homeostatic riboflavin levels and its regulation are very limited. In this study, the
expression pattern of rft-2 at different life stages of C. elegans was studied through real-time PCR, and found
to be consistent from larval to adult stages that demonstrate its involvement in maintaining the body
homeostatic riboflavin levels at whole animal level all through its life. A possible regulation of rft-2
expression at mRNA levels at whole animal was studied after adaptation to low and high concentrations of
riboflavin. Abundance of rft-2 transcript was upregulated in riboflavin-deficient conditions (10 nM), while it
was downregulated with riboflavin-supplemented conditions (2 mM) as compared with control (10 μM).
Further, the 5 -regulatory region of the rft-2 gene was cloned, and transgenic nematodes expressing transcriptional
rft-2 promoter::GFP fusion constructs were generated. The expression of rft-2 was found to be
adaptively regulated in vivo when transgenic worms were maintained under different extracellular riboflavin
levels, which was also mediated partly via changes in the rft-2 levels that directs towards the possible
involvement of transcriptional regulatory events.
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Keywords
BVVLS, C. elegans, ontogeny and adaptive regulation, rft-2, riboflavin transport
Citation
Krishnan Gandhimathi, Sellamuthu Karthi, Paramasivam Manimaran, Perumal Varalakshmi, Balasubramaniem Ashokkumar. Riboflavin transporter-2 (rft-2) of Caenorhabditis elegans: Adaptive and developmental regulation. Journal of Biosciences. 2015 June; 40(2): 257-268.