A high yield DNA extraction method for medically important Candida species: A comparison of manual versus QIAcube‑based automated system.
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Date
2016-10
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Abstract
The prognosis of infected individuals with candidemia depends on rapid and precise diagnosis which enables
optimising treatment. Three fungal DNA extraction protocols have been compared in this study for medically important
Candida species. The quality and quantity of the DNA extracted by physical, chemical and automated protocols
was compared using NanoDrop ND-2000 spectrophotometer. It was found that the yield and purity (260/230) ratio
of extracted DNA was significantly high in the physical treatment‑based protocol as compared to chemical based or
automated protocol. Extracted DNA‑based real time‑polymerase chain reaction showed an analytical sensitivity of
103 cfu/mL. The result of this study suggests physical treatment is the most successful extraction technique compared
to other two protocols.
Description
Keywords
Automated extraction, Candida spp., DNA extraction, manual extraction, real‑time polymerase chain reaction
Citation
Das P, Pandey P, Harishankar A, Chandy M, Bhattacharya S. A high yield DNA extraction method for medically important Candida species: A comparison of manual versus QIAcube‑based automated system. Indian Journal of Medical Microbiology. 2016 Oct-Dec; 34(4): 533-535.