Genetic Variations in Asparagus racemosus, an Endangered Medicinal Herb Endemic to India Using RAPD Markers.
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Date
2016
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Abstract
Aim: To study the genetic diversity in Asparagus racemosus germplasm using RAPD molecular
markers for its better conservation and utilization.
Study Design: RAPD markers used to check genetic diversity in Asparagus racemosus using
different softwares.
Place and Duration of Study: Department of Bio & Nano Technology, Guru Jambheshwar
University of Science & Technology, Hisar-125001 between May 2013 to June 2014.
Methodology: A total of 60 RAPD markers used to check polymorphism at genetic level among
60 asparagus genotypes. PCR amplified bands were scored as 0 and 1 for absence and presence.
The binary data so obtained used to reveal genetic polymorphism via NTSYS, POPGENE and AMOVA analysis.
Results: A significant level of genetic diversity (81.48%) among all genotypes was assessed by
using RAPD molecular markers. Out of 60, 49 RAPD primers produced 425 polymorphic loci. The
value of Jaccard’s coefficient varied from 0.48 to 0.97 for RAPD. OPB-15 primer proved to be the
most polymorphic marker among all used. The POPGENE analysis revealed 44.44, 79.01 and
64.20% polymorphism for RAPD analysis in groups with low, intermediate and high saponin content.
The overall value of Shannon’s index and Nei’s genetic diversity was 0.3402 & 0.2169 for RAPD
marker system.
Conclusion: These results showed RAPD marker system useful in detecting significant genetic
polymorphism among genotypes which can be used for production and conservation of improved
genotypes.
Description
Keywords
Asparagus racemosus, genetic polymorphism, RAPD, endangered medicinal plant
Citation
Kumar Mahesh, Naik Pradeep Kumar, Sarla, Chhokar Vinod. Genetic Variations in Asparagus racemosus, an Endangered Medicinal Herb Endemic to India Using RAPD Markers. British Biotechnology Journal. 2016; 10(2): 1-11.