Do phosphatase of regenerating liver‑3, matrix metalloproteinases‑2, matrix metalloproteinases‑9, and epidermal growth factor receptor‑1 predict response to therapy and survival in glioblastoma multiforme.
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Date
2016-07
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Abstract
Context: Poor survival of the glioblastoma multiforme (GBM) has been
attributed in part to the invasive nature of the lesion making complete surgical
removal near impossible. Phosphatase of regenerating liver‑3 (PRL‑3),
matrix metalloproteinases‑2 and ‑9 (MMP‑2 and MMP‑9), and epidermal
growth factor receptor (EGFR‑1) play a role in invasive nature of tumor cells.
Aims: This study was conducted to evaluate PRL‑3, MMP‑2, MMP‑9, and
EGFR‑1 (markers) expression in cases to GBM and to correlate their expression
with therapy response and survival. Settings and Design: GBM cases
(n = 62) underwent surgery followed by radiation (n = 34) and chemoradiation
(n = 28). Using WHO Response Evaluation Criteria in Solid Tumors criteria
response to therapy was assessed at 3 months and cases followed up for
survival. Subjects and Methods: Expression of markers was assessed by
immunohistochemistry as a percentage of positive tumor cells in hot spots.
Statistical Analysis Used: Kaplan–Meier, ANOVA, Chi‑square test, univariate,
and multivariate Cox‑regression analysis was done. Results: Response to
therapy was evident in 54.8% cases of responders with the mean survival of
494.03 ± 201.13 days and 45.2% cases of non responders (278.32 ± 121.66 days)
with P = 0.001. Mean survival for the patient’s opted chemoradiation was
457.43 ± 222.48 days which was approximately 3 months greater than those
who opted radiation alone (P = 0.029). We found PRL‑3 overexpression was
an independent, significant, poor prognostic factor for survival by multivariate
analysis (P = 0.044). Cases negative for MMP’s and EGFR showed increased
survival, but the difference was insignificant. Conclusion: PRL‑3 expression
appears to be related to an adverse disease outcome.
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Keywords
Epidermal growth factor receptor, glioblastoma multiforme, immunohistochemistry, matrix metalloproteinases, phosphatase of regenerating liver
Citation
Soni Priyanka, Husain Nuzhat, Chandra Anil, Ojha Bal Krishan, Bhatt Madan Lal Brahma, Gupta Rakesh Kumar. Do phosphatase of regenerating liver‑3, matrix metalloproteinases‑2, matrix metalloproteinases‑9, and epidermal growth factor receptor‑1 predict response to therapy and survival in glioblastoma multiforme. Indian Journal of Pathology & Microbiology. 2016 July-Sept 59(3): 287-294.