p53 and p16 in oral epithelial dysplasia and oral squamous cell carcinoma: A study of 208 cases.
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Date
2016-04
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Abstract
Background: The use of p16 and p53 as biomarkers of malignant transformation
of oral epithelial dysplasia (OED) and biological behavior of oral squamous cell
carcinoma (OSCC) is controversial. Aim: To determine the immunoexpression
of p16 and p53 in OED and OSCC and to establish their possible relation to
histopathological grading of OED/OSCC. Materials and Methods: Ninety‑six
OEDs (40 mild, 36 moderate, and 20 severe dysplasia); and 112 OSCCs (64
well‑differentiated, 38 moderately differentiated, and 10 poorly differentiated)
coming from archives of four centers of oral pathology were included. Histological
slides from all cases were processed with immunohistochemical technique
using anti‑p53 and anti‑p16 antibodies. The intensity of the immunoreactivity
were classified using the ImageLab®MCM systemas follows: <60 mild, >60–<90
moderate, and >90 strong. Forstatistical purposesa χ2 test (P < 0.05) was
performed. Results: Severe dysplasia show highest relative frequency of
p16‑positive (35.5%), whereas p53 is associated with mild dysplasia (P = 0.04).
Moderately differentiated OSCC had larger relative frequency of p16‑positive and
p53‑positive cases (47.3% both circumstances) (P > 0.05). Statistical association
of p16‑positive and p53‑positive cells to basal stratum of OED (P = 0.0008;
P = 0.0000, respectively) and p16‑positive cells and p53‑positive cells to
perivascular zone of OSCC (P = 0.001; P = 0.0000, respectively) was found.
Conclusions: p16 and p53 could be not specific enough to identify patients
suffering OED with high risk to malignancy; however, the evaluation of the
presence of p16 and p53 in the tumoral invasive front of OSCC could contribute
to establish the tumor progression.
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Keywords
Oral cancer, oral epithelial dysplasia, oral squamous cell carcinoma, p16, p53
Citation
Gonzalez Juan C Cuevas, Cepeda Luis A Gaitan, Yanez Socorro A Borges, Cornejo Alejandro Donohue, Estevez Ana D Mori, Huerta Elba Rosa Leyva. p53 and p16 in oral epithelial dysplasia and oral squamous cell carcinoma: A study of 208 cases. Indian Journal of Pathology & Microbiology. 2016 Apr-June 59(2): 153-158.