Effect of thiamine pyrophosphate on retinopathy induced by hyperglycemia in rats: A biochemical and pathological evaluation.
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Date
2016-06
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Abstract
Purpose: Information is lacking on the protective effects of thiamine pyrophosphate (TPP) against
hyperglycemia‑induced retinopathy in rats. This study investigated the biochemical and histopathological
aspects of the effect of TPP on hyperglycemia‑induced retinopathy induced by alloxan in rats.
Materials and Methods: The rats were separated into a diabetic TPP‑administered group (DTPG), a diabetes
control group (DCG) and a healthy group (HG). While the DTPG was given TPP, the DCG and HG were
administered distilled water as a solvent at the same concentrations. This procedure was repeated daily for
3 months. At the end of this period, all of the rats were euthanized under thiopental sodium anesthesia, and
biochemical and histopathological analyses of the ocular retinal tissues were performed. The results of the
DTPG were compared with those of the DCG and HG. Results: TPP prevented hyperglycemia by increasing
the amount of malondialdehyde and decreasing endogen antioxidants, including total glutathione,
glutathione reductase, glutathione S‑transferase and superoxide dismutase. In addition, the amounts of the
DNA oxidation product 8‑hydroxyguanine were significantly lower in the retinas of the DTPG compared
to the DCG. In the retinas of the DCG, there was a marked increase in vascular structures and congestion,
in addition to edema. In contrast, little vascularization and edema were observed in the DTPG, and there
was no congestion. The results suggest that TPP significantly reduced the degree of hyperglycemia‑induced
retinopathy. Conclusions: The results of this study indicate that TPP may be useful for prophylaxis against
diabetic retinopathy.
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Keywords
Alloxan, antioxidants, diabetic retinopathy, oxidants, rat, thiamine
Citation
Cinici Emine, Ahiskali Ibrahim, Cetin Nihal, Suleyman Bahadir, Kukula Osman, Altuner Durdu, Coban Abdulkadir, Balta Hilal, Kuzucu Mehmet, Suleyman Halis. Effect of thiamine pyrophosphate on retinopathy induced by hyperglycemia in rats: A biochemical and pathological evaluation. Indian Journal of Ophthalmology. 2016 June; 64(6): 434-439.