Identification of molecular markers to study the Garcinia spp. diversity.
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Date
2016-06
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Abstract
The genus Garcinia shows a considerable variation in its morphological characters such as leaf, flower and fruit with taxonomic ambiguity. It is a potential under-exploited multipurpose crop that gained considerable attention for the presence of (-) hydroxycitric acid, an anti-obesity compound, in its fruit rind and leaves. Here, we evaluated the genetic relationship through molecular markers among the selected 9 species commonly available in the Western Ghats and the Northeastern Himalayan foot hills of India. The nucleotide sequence data obtained from two prominent monomorphic bands generated in ISSR profiling of the species was utilized for the study. The selected bands were found to be of ITS region (700 bp) and partial region of KNOX-1 gene (600 bp). The evolutionary cluster was formed using MEGA5 software. The study indicated 2 major clusters, influenced by floral morphology of the species and availability of (-) hydroxycitric acid in their fruit rinds. In the subclusters, one species from the Western Ghats were paired with another from Northeastern Himalayas with relatively similar morphological traits.
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Keywords
ITS region, ITS region KNOX-1 gene, Northeastern Himalayas, Species diversity, Western Ghats
Citation
Parthasarathy, Utpala Nandakishore, OP Rosana, OB babu, Nirmal K Kumar, Senthil R Parthasarathy, VA. Identification of molecular markers to study the Garcinia spp. diversity. Indian Journal of Experimental Biology. 2016 June; 54(6): 400-405.