Variation in Growth Pattern and Morphological Appearance of Primary Monolayer Cultures of Chondrocytes and Neural Cells Isolated from the Chick Embryo at Different Stages.
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Date
2015-07
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Abstract
Aim: This study was an attempt at investigating the variation in growth and morphology of primary avian chondrocyte and neural cell
cultures established under standard laboratory conditions. Methods: For establishing chondrocyte cultures, the tibia were dissected
from chick embryos that were 12 (E12), 14 (E14) and 15 (E15) days old. For the neural cell culture initiation, the two lobes of the
forebrain were dissected from chick embryos that were 4 (E4), 8 (E8) and 12 (E12) days old. The final characterization of the cells
was performed by H&E and CFV staining. Results: In the chondrocyte cultures, two forms of morphology were observed which is
reversible in process.The primary variation evaluated in chondrocytes cultures was the effect of the media on the state of the cells.
Based on whether the cells were cultured in DMEM or MEM, there was a difference in the transformation of the attached cells from
the differentiated to de-differentiated forms. The primary variation examined in neuron cultures was the embryonic age of the tissue
and the effect that it had on the proliferation and neurite formation of the cells. E8 embryo neural cells cultures result in well-developed
cells with neurite formation along with axonal and dendritic outgrowths with highly complex interconnections between them.
Conclusion: Ultimately, this study demonstrated the composition of culture media had an effect on the morphological appearance
of the chondrocytes, as well as, confirmed that the culture of primary neurons is best performed using cells from an E8 embryo.
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Keywords
Primary monolayer culture, Chondrocytes, Neural cells, cell morphology, Characterization
Citation
Shrestha Rupendra, Palat Asha, Anbarasan Selvam, Paul Solomon F D. Variation in Growth Pattern and Morphological Appearance of Primary Monolayer Cultures of Chondrocytes and Neural Cells Isolated from the Chick Embryo at Different Stages. Acta Medica International. 2015 July-Dec; 2(2): 61-67.