Study about Genetic Variability of SE33 Locus.
Loading...
Date
2015-07
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
Introduction: The HUMACTBP2 (SE33) locus is one of the most polymorphic markers commonly used in forensic human
identification. Variability of SE33 was studied in 2 Calabria and Malta populations using the AmpFlSTR NGM SElect™ PCR
Amplification Kit (Applied Biosystems) and the PowerPlex ESI 17 (Promega). Material & Methods: A total of 41 different alleles were
observed in the 2 examined populations with no allele being more frequent than 10,5%. In the Maltese population more intermediate
alleles than in Calabria were found. Allelic frequencies and statistical parameters of forensic interest (Dp,PE, RMP) were calculated
using PowerStats v.1.2 software. Hardy-Weinberg equilibrium and other population parameters were calculated using Arlequin
v.3.1 and TFPGA v1.3 softwares. Results: No significant deviations from Hardy–Weinberg equilibrium were found. Calabria and
Malta allelic frequencies were compared to previously published population data and no significant differences were found. When
comparing with Sicily no overall significant genetic distances were found, while comparison to other populations showed significant
ones. Moreover comparison with non-European population showed no big distances between Germany and Morocco and between
Hungary and Turkey. Conclusion: confirmed the locus is effectively highly polymorphic and useful not only for forensic identification
but also in paternity cases in addition to the set of STRs loci commonly used..
Description
Keywords
Allele, Hardy–Weinberg equilibrium, SE33 Locus
Citation
Barbaro A, Cassar M, Cormaci P, Grech J C. Study about Genetic Variability of SE33 Locus. Acta Medica International. 2015 July-Dec; 2(2): 16-18.