Distribution of Class II integrons and their contribution to antibiotic resistance within Enterobacteriaceae family in India.
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Date
2016-07
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Abstract
Background: Integrons are the main contributors to the development of multidrug resistance (MDR) among
Gram‑negative bacilli. There is a lack of knowledge about the molecular relation between gene cassettes and
antibiotic resistance in India. Objective: In this study, we have investigated the occurrence of Class II integron
and their cassette array among Enterobacteriaceae. Materials and Methods: A total of 268 MDR non‑duplicate
strains of Enterobacteriaceae were collected from Silchar Medical College and Hospital, Silchar, Assam, India,
during June 2012 to May 2013. Polymerase chain reaction was performed for detection of the integrase genes
and gene cassettes within the Class II integron which were further analysed by sequencing. Results: Class II
integron was observed in 47 isolates. Four different gene cassette arrangements were detected: dfrA1‑sat2‑aadA1;
dfrA1‑sat2‑aadA1‑orfX‑ybeA‑ybfA‑ybfB‑ybgA; dfrA12‑sat2‑aadA1; and dfrA1‑linF‑aadA1. The most prevalent cassette
combination was dfrA1‑sat2‑aadA1. This study has also identified a set of gene cassette associated with linF gene
instead of sat2 gene. Conclusion: Further investigation is required to determine the current situation and important
reservoir of Class II integron for the transmission of drug resistance among Enterobacteriaceae and their contribution to
antimicrobial resistance in hospital environment.
Description
Keywords
Class II integron, Enterobacteriaceae, gene cassette, integrase, multidrug resistance
Citation
Singha P, Chanda D D, Maurya A P, Paul D, Chakravarty A, Bhattacharjee A. Distribution of Class II integrons and their contribution to antibiotic resistance within Enterobacteriaceae family in India. Indian Journal of Medical Microbiology. 2016 July-Sept; 34(3): 303-307.