Typing of Plasmodium falciparum DNA from 2 years old Giemsa‑stained dried blood spots using nested polymerase chain reaction assay.
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Date
2016-04
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Abstract
A panel of 129 Giemsa‑stained thick blood spots (TBS) confirmed for Plasmodium falciparum infection having
different levels of parasite density were collected from a malaria endemic area. DNA was extracted and nested
polymerase chain reaction (PCR) assay was performed to amplify P. falciparum DNA. Nested PCR assay successfully
amplified P. falciparum DNA at a very low parasitaemia of ~10 parasites/μl of blood. Current PCR assay is very simple
and can be used retrospectively to monitor the invasion and prevalence of different Plasmodium species in endemic areas.
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Keywords
Parasitaemia, Plasmodium, polymerase chain reaction, thick blood spots
Citation
Kumar D, Dhiman S, Rabha B, Goswami D, Yadav K, Deka M, Veer V, Baruah I. Typing of Plasmodium falciparum DNA from 2 years old Giemsa‑stained dried blood spots using nested polymerase chain reaction assay. Indian Journal of Medical Microbiology. 2016 Apr-June; 34(2): 210-212.