Loop mediated isothermal amplification assay using hydroxy naphthol blue, conventional polymerase chain reaction and real-time PCR in the diagnosis of intraocular tuberculosis.
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Date
2015-10
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Abstract
This study is a comparative evaluation (Chi-square test) of a closed tube loop mediated isothermal amplification assay
using hydroxy naphthol blue dye (HNB-LAMP), real-time polymerase chain reaction (PCR) and conventional PCR
in the diagnosis of intraocular tuberculosis. Considering clinical presentation as the gold standard in 33 patients, the
sensitivity of HNB-LAMP assay (75.8%) was higher (not significant, P value 0.2) than conventional PCR (57.6%)
and lower than real-time PCR (90.9%). Specificity was 100% by all three methods. No amplification was observed in
negative controls (n = 20) by all three methods. The cost of the HNB-LAMP assay was Rs. 500.00 and it does not
require thermocycler, therefore, it can be used as an alternative to conventional PCR in resource-poor settings.
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Keywords
Hydroxynaphthol blue, loop mediated isothermal amplification assay, MPB64, ocular tuberculosis, real-time polymerase chain reaction
Citation
Balne P K, Basu S, Rath S, Barik M R, Sharma S. Loop mediated isothermal amplification assay using hydroxy naphthol blue, conventional polymerase chain reaction and real-time PCR in the diagnosis of intraocular tuberculosis. Indian Journal of Medical Microbiology. 2015 Oct-Dec; 33(4): 568-571.