Mebiolgel, a thermoreversible polymer as a scaffold for three dimensional culture of Huh7 cell line with improved hepatocyte differentiation marker expression and HCV replication.
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Date
2015-10
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Abstract
Purpose: A novel three dimensional (3D) culture system purely synthesised from co‑polymer which is free from
biological contamination for Huh7 cell cultivation and hepatitis C virus (HCV) replication has been attempted.
Materials and Methods: Mebiolgel, a thermo‑reversible gelation polymer was used as a 3D scaffold for culturing
Huh7, a liver carcinoma cell line used in our study. The 3D culture of the cells were infected with cell culture derived
HCV. Result: The scaffold supported the cell growth as 3D spheroids for up to 63 days. Moreover mebiolgel was found
to be improving the hepatocyte differentiation of Huh7 cells at the transcript level. Three dimensional culture was
susceptible for HCV infection, and this was confirmed by detecting the HCV replication intermediate viral core antigen.
Conclusion: Mebiolgel based culture system was proven to be suited for 3D culture of Huh7 cells by improvising liver
specific genotypic expression and was susceptible for HCV replication. Since mebiolgel based Huh 7 express better
hepatocyte differentiation markers genotypically, this can be implemented as an alternate for primary hepatocytes in
studies such as viral isolation from patient serum.
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Keywords
Hepatitis C virus, hepatocyte, Huh7, mebiolgel, three dimensional culture
Citation
Rajalakshmy A R, Malathi J, Madhavan H N, Samuel J K A. Mebiolgel, a thermoreversible polymer as a scaffold for three dimensional culture of Huh7 cell line with improved hepatocyte differentiation marker expression and HCV replication. Indian Journal of Medical Microbiology. 2015 Oct-Dec; 33(4): 554-559.