How to develop an in-house real-time quantitative cytomegalovirus polymerase chain reaction: Insights from a cancer centre in Eastern India.
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Date
2015-10
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Abstract
Development of a reliable, cost-effective cytomegalovirus quantitative polymerase chain reaction (QPCR) is a priority
for developing countries. Manufactured kits are expensive, and availability can be inconsistent. Development of an
in-house QPCR kit that is reliable and quality assured requires significant effort and initial investment. However, the
rewards of such an enterprise are manifold and include an in-depth understanding of molecular reactions, and expertise
in the development of further low-cost molecular kits. The experience of an oncology centre in Eastern India has been
shared. Hopefully, this would provide a brief roadmap for such an initiative. Staff with adequate understanding of
molecular processes are essential along with vital infrastructure for molecular research and development.
Description
Keywords
Cytomegalovirus, standardisation, troubleshooting, validation, viral load
Citation
Harishankar Anusha, Chandy Mammen, Bhattacharya Sanjay. How to develop an in-house real-time quantitative cytomegalovirus polymerase chain reaction: Insights from a cancer centre in Eastern India. Indian Journal of Medical Microbiology. 2015 Oct-Dec; 33(4): 482-490.