Clinical Outcomes Associated with Interleukin 6 and Interleukin 8 Cytokine Production in Cystic Fibrosis.
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Date
2015
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Abstract
Background: Cystic fibrosis (CF) clinical outcomes remain difficult to prognosticate. Elevated
cytokine profiles in vivo are associated with obstructive lung diseases including CF. However, no
studies to date have conducted a longitudinal clinical outcome analysis of early cytokine response
on CF.
Materials: To assess high risk cystic fibrosis (CF) clinical outcomes based on peripheral blood
mononuclear cell (PBMC) in vitro cytokine response to Pseudomonas auruginosa antigen.
Methods: PBMCs were stimulated with Pseudomonas aeruginosa antigen. Resultant cytokines
TNF-alpha, IL-6 and IL-8 were measured by ELISA. PBMC cytokine response was characterized
as high and low production of cytokines. Each group was analyzed for subsequent clinical
outcomes including pulmonary function and rates of hospitalization.
Results: Low and high level TNF-alpha and IL-6 groups lung function rate of decline did not show
any remarkable difference. Between the groups with low and high levels of IL-8, there was a significant difference in rates of decline of FVC and FEV-1, with a greater decline in the high IL-8
producing group than the low IL-8 group.
Conclusion: Stimulated cytokine production in vitro was associated with specific long-term clinical
outcomes of CF patients. Specifically, elevated IL-8 production after Pseudomonas aeruginosa
stimulation correlated with decline in lung function and hospitalization rate over 14 years of clinical
follow up. This approach may potentially limit cytokine impact on pulmonary disease burden.
Description
Keywords
Cystic fibrosis, interleukin, cytokine, Pseudomonas
Citation
Randhawa Inderpal S, Nussbaum Eliezer M, Ahdoot Rebecca A, Rees Hannah M, Yu Albert W , Cihn Terry W. Clinical Outcomes Associated with Interleukin 6 and Interleukin 8 Cytokine Production in Cystic Fibrosis. British Journal of Medicine and Medical Research. 2015; 6(2): 164-172.