MMP-9, MMP-2 Profile in Different Mice Tissues and LPS Induced MMP-9 and MMP-2 Expression in RAW 264.7 Cells, Down Regulated by Emodin Treatment.
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Date
2014-09
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Abstract
Emodin, an anthraquinones component of Rheum palmatun, has been used for anti-inflammatory
purposes. However, its underlying molecular effect(s) on target cells remain to be well clarified. our
current study was aimed at investigating the regulatory effect of Emodin on lipopolysaccharide induced
inflammatory responses in RAW 264.7 macrophages by gelatin zymography . It was found that treatment
of LPS(5μg/ml) for 18 h elevates the expression of MMP-9 and MMP-2 proteins in RAW 264.7 cells. It
was also found that co-treatment of RAW 264.7 with drug Emodin(6μg/ml) and LPS(5μg/ml) for the
period of 18 h reduces the expression level of MMP-9 and MMP-2 protein. Our data suggest that Emodin
plays roles in regulating the digestion of Extracellular matrix by down-regulating the expression of
MMP-9 and MMP-2 proteins.
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Keywords
MMP-9, MMP-2, RAW 264.7 cell line, lipopolysaccharide,
Citation
Lone Bilal Ahmad. MMP-9, MMP-2 Profile in Different Mice Tissues and LPS Induced MMP-9 and MMP-2 Expression in RAW 264.7 Cells, Down Regulated by Emodin Treatment. International Journal of Interdisciplinary and Multidisciplinary Studies. 2014: Sept; 1(9) 1-7.