Research of HLA II Class DRB1, DQA1, DQB1 Genetic Markers in Patients with HIV Infection and AIDS.
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Date
2014-09-21
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Abstract
Aims: To find out the most frequent associations of the HLA II class loci DRB1*, DQA1*,
DQB1* with the HIV/AIDS infection.
Place and Duration of Study: The study took place in The Laboratory of Clinical
Immunology and Immunogenetics (LCII) of Riga Stradiņš University (RSU), Riga, Latvia,
Riga Eastern Clinical University Hospital, “Infectology Centre of Latvia”, between May
1991 and December 2004.
Original Research Article
British Journal of Medicine & Medical Research, 4(17): 4482-4500, 2014
4483
Methodology: We analysed the medical documentation of 2500 patients and included
1180 (888 men, 292 women, 185 of them in AIDS phase) HIV infected patients. Genomic
DNA was extracted from the blood with phenol-chloroform extraction method. Lowresolution
HLA typing for HLA- DRB1*; DQB1*; DQA1* was performed by polymerise
chain reaction (PCR) with amplification with sequence-specific primers (SSP). PCR
products were separated on 3% agarose, the amplified bands were visualized, and the
HLA-DRB1; DQA1; DQB1 type was deduced.
Results: Genetic markers of immunologic alleles upon development of HIV infection –
HLA-DRB1*03(17:01); 05(11:01); 07:01; HLA-DQA1*01:01; 02:01; 03:01; 06:01; HLADQB1*
03:02; 05:01; 03:03; 03:04, as well as resistance markers connected with slow
development of HIV infection – HLA-DRB1*01:01; 04:01; 06(13:01); HLA-DQA1*01:03;
04:01; 05:01; HLA-DQB1*03:01; 03:03; 04:01-2; 06:01; 06:02-8 are located in different
groups of patients. High risk markers in case of HIV infection development belonging to
the following groups of alleles: HLA-DRB1*03(17:01), DRB1*05(11:01), DQA1*01:01;
03:01 un DQB1*05:01; 03:02, as well as three-loci haplotypes HLA-DRB1*03(17:01)/
DQB1*05:01/ DQA1*01:01; HLA-DRB1*05(11:01)/DQB1*03:01/ DQA1 *05:01;
DRB1*01:01/DQB1*03:02/ DQA1*03:01 and DRB1*01:01/DQB1*05:01/DQA1*01:01 are
determined. Resistance to HIV infection development forms in the following groups of
alleles: HLA-DRB1*01:01; 06(13:01), HLA-DQB1* 03:01; 06:02-8; HLA-DQA1*01:02;
01:03, as well as in haplotypes HLA- DRB1*01:01/DQB1*06:02-8/DQA1*01:02;HLADRB1*
06(13:01)/DQB1*06:02-8/DQA1*01:02; HLADRB1*
01:01/DQB1*03:01/DQA1*01:02; and HLA-DRB1*06(13:01)/DQB1*06:02-8/
DQA1*01:02 in different groups of HIV/AIDS patients.
Conclusion: The prevalence of genes DRB1; DQA1; DQB1 and DRB1-DQA-DQB1
combinations in the five groups of HIV infected patients have been established.
Comparative analysis was performed also in the group of healthy donors (control group).
The role of the main histocompatibility complex has been established, it enables marker
functions and that can be used in the additional prognostic diagnostics in case of HIV
infection. The obtained results testify that upon the identification of HIV genes it is possible
to understand the molecular mechanisms in case of progression of AIDS syndrome
complex; this possibly can be beneficial for the determination of the clinical results of
infected patients.
Description
Keywords
HIV, AIDS, DRB1;, DQA1, DQB1
Citation
Kasjko Diana, Jasinskis Vladislavs, Eglite Jelena, Golushko Jelena , Sture Gunta, Kalimulin Arthur, Sochnevs Arthur, Viksna Ludmila.Research of HLA II Class DRB1, DQA1, DQB1 Genetic Markers in Patients with HIV Infection and AIDS. British Journal of Medicine and Medical Research. 2014 Sept; 4(27): 4482-4500.