Association of BRCA1/2 Mutation and TP53 Over-Expression with Sporadic vs. Hereditary Iraqi Ovarian Cancer Patients.
Loading...
Date
2014-05-11
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
Aims: In Iraq, ovarian cancer is the fifth most common cause of death, and the 6th in the
list of most common cancers. While TP53 is the most common tumor suppressor gene
involved with human malignancies, ovarian cancer is already known to be linked to the
variations in the breast cancer genes, BRCA1 and BRCA2. Herein we aim to estimate
the rate of BRCA1, BRCA2 gene mutation and TP53 immuno-expression in patients with
hereditary vs. sporadic ovarian cancer and to show the correlation of these biomarkers to
some clinicopathological parameters.
Study Design: This is a correlational case-control study design.
Place and Duration of Study: The present study was performed in the Department of
microbiology; Genetic section, College of Medicine, Babylon University. Samples taken
from patients referred to general teaching hospitals and some of the private laboratories
in Al-Hilla and Al-Najaf governorate, in the middle of Iraq, over a period from January
2013 to November 2013.
Methodology: Fifty-eight patients with ovarian carcinoma (30 sporadic and 28
hereditary), their ages ranging between 28-77 years, and thirty healthy women as control
were included in this study. Genetic study using PCR technique was employed for
BRCA1/2 gene mutation detection. Avidin-Biotin Complex (ABC) method was employed
for immune-histochemical detection of Tp53 gene over-expression.
Results: BRCA1l2 gene mutation was found in 21 and 13 cases out of 28 hereditary and
30 sporadic ovarian cancer cases respectively. TP53 over-expression was detected in
18 and 17 cases out of 28 hereditary and 30 sporadic ovarian cancer cases. BRCA1l2 gene mutation and TP53 over-expression was reported more frequently in higher stage
of tumor (P=0.05). The large majority of cases were diagnosed in later ages, patients
with sporadic cancer got the disease later than hereditary ones.
Conclusion: BRCA1/2 and Tp53 genes alterations appears to be more important in
hereditary than in sporadic ovarian cancer pathogenesis and evolution, as they are
frequently associated with biologically aggressive tumors (high stages). Furthermore,
TP53 gene over-expression was found to be more correlated with the cancer occurrence
than BRCA1/2 mutation.
Description
Keywords
BRCA1l2 gene, TP53 gene, ovarian carcinoma, sporadic, hereditary, Iraqi patients
Citation
Alshibly Ifad Kerim. Association of BRCA1/2 Mutation and TP53 Over-Expression with Sporadic vs. Hereditary Iraqi Ovarian Cancer Patients. British Journal of Medicine and Medical Research. 2014 May; 4(14): 2684-2693.