Panax ginseng C.A. Meyer Extract Protects Rat Erythrocyte from the Oxidative Damage Induced by the Synergistic Effects of Subchronic Treatment with Aflatoxin B1 and Fumonisin.
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Date
2014-03-21
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Abstract
Aims: The current study aimed to evaluate the protective role of ginseng extract (GE)
against the oxidative damage of erythrocytes resulted from the synergistic effects of
subchronic (84 days) treatment with aflatoxin B1 (AFB1) and fumonisin B (FB) in male
and female rats.
Study Design: Animals were divided into six groups of ten rats (5 males and 5 females)
included: the control group; GE alone-treated group (150 mg/kg b.w); the group treated
orally with AFB1 (17 μg/kg b.w) during the first 2 weeks and fed FB1-contaminated diet
(100 mg/kg diet) during the 6th to 8th weeks; the group treated with GE during the mycotoxin protocol and continued till week 10; the group treated with GE 2 weeks before
AFB1 administration and continued till the end of FB1 treatment and the group treated
with GE for 4 weeks after the toxin protocol stopped.
Methodology: Blood samples were collected at the end of treatment for hematological
and biochemical determinations.
Results: The results showed that the mycotoxins ingestion caused insignificant
decrease in total hemoglobin (Hb) content, whereas they caused a significant increase in
contents of Met-Hb, Carboxy-Hb and Sulf-Hb fractions accompanied by a significant
decrease in Oxy-Hb faction, compared to the control group. Erythrocyte Met-HbR, SOD
and GR activities were significantly decreased after mycotoxins treatment. In addition,
mycotoxins ingestion induced a significant decrease in serum levels of iron and TIBC but
did not significantly affect serum ferritin level. These effects were pronounced in male
rats than in females. Treatment with GE ameliorated the erythrocytes damage induced
by mycotoxins as indicated by the modulations in most of the investigated markers.
Conclusion: Treatment with GE has beneficial effect in counteracting erythrocyte
membrane damage and hemoglobin changes induced by aflatoxin B1 and fumonisin B1.
This effect may be probably through its potent antioxidative activity.
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Keywords
Aflatoxin, fumonisin, mycotoxins, oxidative stress, erythrocytes, ginseng
Citation
Abdel-Wahhab Khaled G, Mannaa Fathia A, Abdel-Wahhab Mosaad A. Panax ginseng C.A. Meyer Extract Protects Rat Erythrocyte from the Oxidative Damage Induced by the Synergistic Effects of Subchronic Treatment with Aflatoxin B1 and Fumonisin. British Journal of Medicine and Medical Research. 2014 Mar; 4(9): 1883-1901.