Bromocresol Dye-binding Methods Underestimate Serum Albumin Value in Patients Treated with High Dose of Penicillin G.
Loading...
Date
2014-02-21
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
Background: Various factors contribute to the discrepancies observed between the
bromocresol green (BCG) and bromocresol purple (BCP) assays of serum concentration.
Either a BCG or a modified BCP assay is a routine laboratory for albumin measurement
in Japan. High-dose of penicillin G underestimates serum albumin level using a modified
BCP method in vitro. Therefore, we examined the serum albumin level in the patients
treated with high-dose of penicillin G and also performed the experiments on coincubation
with plasma, or albumin product and penicillin G solution in vitro.
Methods: The medical records of 71 patients treated with high-dose of Penicillin G
collected between 2009 and 2012 were reviewed for age, gender, biochemistry (total
protein, albumin and potassium), underlying diseases and usage of albumin product.
Patients were divided into 2 groups: BCG group (N = 38) and a modified BCP group (N =
33). We compared serum albumin levels between two groups. We performed the experiments on co-incubation with albumin product or human plasma and penicillin G
solution in vitro.
Results: Serum albumin levels using a modified BCP assay decreased during the
treatment with high dose penicillin G (-0.4 ± 0.1 g/dL), while serum albumin levels by a
BCG method did not decrease (0.06 ± 0.05 g/dL). Although only one patient revealed
hypoalbuminemia (<2.0 g/dL) by a BCG method (2.6%), ten patients revealed
hypoalbuminemia by a modified BCP method (33%). Penicillin G underestimated plasma
albumin levels using a modified BCP methods (% underestimation: 42.9 ± 0.0%) more
than those using a BCG method (% underestimation: 10.6 ± 0.0%) in co-incubation
experiments in vitro.
Conclusion: High-dose of penicillin G might cause the underestimation of serum
albumin levels using bromocresol dye-binding methods.
Description
Keywords
Underestimation, hypoalbuminemia, antibiotics, infectious endocarditis
Citation
Fujita Hiroshi, Shiotani Yoko, Ohnishi Kenji, Nishimura Shigeko. Bromocresol Dye-binding Methods Underestimate Serum Albumin Value in Patients Treated with High Dose of Penicillin G. British Journal of Medicine and Medical Research. 2014 Feb; 4(6): 1293-1300.