Catalyzed Mediator-Based Decolorization of Five Synthetic Dyes by Pleurotus ostreatus ARC280 Laccase.
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Date
2015
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Abstract
Aim: The aim of the present study was to evaluate qualitatively the decolorization of five dyes by
Pleurotus ostreatus (P. ostreatus) ARC280 using solid medium. The laccase produced by the
fungus was used in terms of its concentration and thermal stability for enzymatic decolorization and
also in combination with Hydroxybenzotriazole (HBT) as a redox mediator.
Study Design: Qualitative evaluation of decolorization of dyes and determining the best conditions
required for decolorization in the presence and absence of HBT.
Place and Duration of Study: Department of Microbial Chemistry, Genetic Engineering and
Biotechnology Division, National Research Centre (NRC), Cairo, Egypt, between January 2013 and
February 2014.
Methodology: P. ostreatus ARC280 fungal ability for dyes decolorization was qualitatively
evaluated on solid medium containing (g/L): dye, 0.1; glucose, 10; agar, 30; 100 mL mineral
solution and 100 mL wheat bran washing water obtained by boiling 50 g of wheat bran in 1000 mL of distilled water. The efficiency of decolorization was expressed in terms of decolorization
percentage as follows:
Decolorization (%) = 100 ×
Absorbance t0 - Absorbance tf
Absorbance t0
Where Absorbance t0 is the absorbance at the optimumwavelength of the reaction mixture before
incubation with the enzyme and Absorbance tf is the absorbance at the optimum wavelength after
incubation time.
Results: The enzyme was efficient in decolorizing Acid Blue C.I. 220 (100%), Dichlorophenol
indophenol sodium salt D 5110 (92.6%) and Brilliant Green C.I. 42040 (78.6%) after 6 h of
incubation at 30ºC. In the presence of HBT (1 mM), Lanasol Red 6G was greatly affected by HBT
as a laccase mediator system with decolorization percentage of 53.85% instead of 10.90 in case of
laccase alone, however the enzyme could not efficiently decolorize Foron Yellow Brown S 2RFLI
dye even in presence of HBT. The decolorization efficiency of all dyes was decreased by
increasing reaction temperature from 30 to 50ºC. The absorbance reduction at the maximum
wavelength was recorded with all the tested dyes.
Conclusion: The results obtained clearly confirmed the role of P. ostreatus ARC280 laccase and
its mediated system in the decolorization of structurally different dyes.
Description
Keywords
Pleurotus ostreatus laccase, synthetic dyes, decolorization, hydroxybenzotriazole
Citation
Elshafei Ali M, Othman Abdelmageed M, Hassan Mohamed M, Haround Bakry M, Elsayed Maysa A, Farrag Ayman A. Catalyzed Mediator-Based Decolorization of Five Synthetic Dyes by Pleurotus ostreatus ARC280 Laccase. British Biotechnology Journal. 2015 9(2): 1-15.