In vitro Plantlet Regeneration of Four Local Garlic (Allium sativum) Accessions of Bangladesh.
Loading...
Date
2015
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
Aims: The genetic improvement of garlic can be achieved by biotechnological manipulations as
breeding in this vegetatively propagated crop is limited. The current research was conducted with a
view to develop an efficient in vitro regeneration protocol for four local garlic accessions namely,
G121, G122, G123 and G124.
Place, Duration and Design of Study: The experiment was conducted in the Tissue Culture
Laboratory of the Department of Genetics and Plant Breeding, Bangladesh Agricultural University
during the period from June 2013 to June 2014 using three-factorial experimental design.
Methodology: The root tips, basal disc and leaf base were cultured in MS medium supplemented
with 2, 4-Dichlorophenoxyacetic acid (2, 4-D) alone, and with both 2, 4-D and 6-Benzylaminopurine (BAP) together for callus induction and the later for subsequent sub-culturing and proliferation of
callus. MS medium supplemented with 1-Naphthaleneacetic acid (NAA) and BAP was used for
plantlet regeneration.
Results: The percentage of callus induction increased with the increase in the concentration of 2,4-
D, starting from 0.5 mg L-1 till 2.0 mg L-1 and declined with further increase in the concentration of
2,4-D. The MS medium supplemented with 2,4-D and BAP showed higher percentage of callus
induction and callus proliferation compared to that of with 2,4-D alone. The highest percentage of
callus induction was observed in the genotype G124 from the explant basal disc (85%) and in the
genotype G121 from the explant leaf base (80%) with 2.0 mg L-1 2,4-D and 2.0 mg L-1 BAP. MS
medium supplemented with 2 mg L-1 2,4-D + 0.5 mg L-1 BAP showed highest percentage of callus
proliferation (90%) in almost all the genotypes. The highest percentage of plantlet regeneration
were observed in the genotype G124 for the explants basal disc (63.33%) in MS medium
supplemented with 2 mg L-1 NAA + 1 mg L-1 BAP. The survival rate of the plantlets after
acclimatization varied from 40% (in G123) to 70% (in G121).
Conclusion: The optimized protocol of plant regeneration from local garlic accessions will be
useful for any future garlic improvement programs using biotechnological means.
Description
Keywords
Garlic, tissue culture, callus induction, growth medium, phytohormone
Citation
Haider Sayed Raihanul, Hossain Mohammad Rashed, Rahman Shanjida, Sultana Shirin, Quddus Tamanna, Chakraborti Moutoshi, Hoque Aunamika, Shahriar Md Hasan, Haque Md Ashraful. In vitro Plantlet Regeneration of Four Local Garlic (Allium sativum) Accessions of Bangladesh. British Biotechnology Journal. 2015 8(3): 1-12.