Histopathological Alterations Induced by Naja naja Crude Venom on Renal, Pulmonary and Intestinal Tissues of Mice Model.
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Date
2015
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Abstract
Aim: Snake bite causes a significant number of mortality and morbidity throughout the world. So,
the current study was carried out to estimate the extinct of damage caused by intraperitoneal
introduction of cobra venom on kidney, lung and intestinal tissues of mice model using histological
technique.
Place and Duration of Study: The entire study including the treatment along with preparing
histological slide was conducted in protein science laboratory, Department of Genetic Engineering
and Biotechnology, University of Rajshahi, Bangladesh between December 2013 to July 2014.
Methods: Twenty five mature female albino mice were divided mainly into two groups as control
and envenomated group. Lyophilized Naja naja venom was dissolved in 0.9% NaCl solution and
injected intraperitoneally into the mice of the envenomated group at dosages equivalent to LD50 (0.25 mg/kg). Whereas the animals from control group were not received any venomous
component. Both groups of animal were sacrificed for histological study and visualized under light
microscope.
Results: Injection of cobra venom induced a range of histological changes in all envenomated mice
comparing with their control. Results from the histopathological examination showed mainly
inflammatory cellular infiltration, vacuolation in renal tubules, shrinking of glomeruli, raising space
between the walls of Bowman’s capsule in renal tissue and alveolar haemorrhage, inflammatory
cellular infiltration and edema in pulmonary tissue. No significant histopathological alterations in
intestinal tissue were observed without infiltration and mild hemorrhage.
Conclusion: The findings from the current study revealed that, cobra venom at lethal dose causes
multiple organ failure in experimental animal which could be considered among the factors that lead
to death. By observing the site and the mode of action on tissue level, these findings may help to
allay the severity of damage by discovering novel anti venom drug.
Description
Keywords
Cobra, histology, intestine, kidney, lung, Mus musculus and venom
Citation
Al- Mamun M A, Hakim M A, Zaman M K, Hoque K M F, Ferdousi Z, Reza M Abu. Histopathological Alterations Induced by Naja naja Crude Venom on Renal, Pulmonary and Intestinal Tissues of Mice Model. British Biotechnology Journal. 2015 6(3): 119-125.