Response Surface Methodology Based Optimization of a New Isolate Bacillus pumilus ZR LS S2 with Fibrinolytic Activity.
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Date
2015
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Abstract
Aims: The work aims to isolate potential fibrinolytic enzyme producing isolates from various
samples with the aim of developing a suitable optimization strategy using response surface
methodology.
Study Design: Plackett- Burman and Face centered central composite design was used.
Place and Duration of Study: Department of Life Sciences, University of Calicut.
Methodology: Various samples were screened using fibrin agar plates for the isolation of
fibrinolytic enzyme producing bacteria and was identified by 16 s rRNA sequencing. Further the
physical parameters and media components were optimized using Plackett- Burman design and
face centered central composite design.
Results: A novel isolate with fibrinolytic potential was isolated and was identified as Bacillus
pumilus ZR LS S2. A novel media was formulated using this data, for the production of fibrinolytic
enzyme, which contains peptone, casein, MgSO4 and NaCl. The isolate produced 6738.384 U/ml of
enzyme in optimized conditions.
Conclusion: Initial studies were performed for the production of the fibrinolytic enzyme by the
novel isolate, further studies are required to effectively validate the potential of the fibrinolytic
enzyme and to develop effective application for the same.
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Keywords
Bacillus pumilus, response surface methodology, fibrinolytic enzyme
Citation
Reshma C V, Zuhara K Fathimathu. Response Surface Methodology Based Optimization of a New Isolate Bacillus pumilus ZR LS S2 with Fibrinolytic Activity. British Biotechnology Journal. 2015 6(2): 51-61.