Production and Partial Characterization of Pectinase from Snail (Archachatina marginata) Gut Isolates.
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Date
2015
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Abstract
Aim: To identify isolates for industrial production of pectinase.
Methodology: The isolates were screened for pectinolytic activity using pectin as substrate.
Enzyme activity was expressed as mg of glucose equivalent released per ml of crude enzyme
solution per second. The kinetic parameters of the enzyme were determined to obtain the optimum
pH, temperature, Km and Vmax. Pectinase produced from Bacillus subtillis was immobilized on
chitosan and its activity was compared with that of free enzyme.
Results: Pectinase from Bacillus subtilis was screened to have the highest enzymatic activity
among bacterial isolates while pectinase from Aspergillus niger had the highest enzymatic activity
among fungal isolates. High yield of pectinase enzyme was obtained from B. subtilis after 24hrs
with activity of 4.01×10-4 mg/ml/sec while high yield of pectinase was obtained from A. niger on the
5th day with activity of 2.07×10-4 mg/ml/sec. The optimum pH for pectinase produced from B. subtilis
and A. niger were 8 and 6, respectively. The optimum temperature for pectinase from B. subtilis
and A. niger were at 50ºC and 40ºC, respectively. The Vmax and Km of pectinase from B. subtilis and A. niger were 16.88×10-4 (mg/ml/sec) and 10 (mg/ml); 9.29×10-4 (mg/ml/sec) and 30 (mg/ml),
respectively. Optimum temperature and pH of immobilized pectinase were 70ºC and 4.0,
respectively. Residual activity of immobilized enzyme was 92% after storage at 4ºC for 14 days.
Conclusion: This study revealed that Bacillus subtilis from snail gut may be considered as a good
candidate for industrial production of pectinase.
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Keywords
Snail gut isolates, pectinase, enzyme activity, Km and Vmax, immobilized pectinase
Citation
Egwim E C, Caleb T. Production and Partial Characterization of Pectinase from Snail (Archachatina marginata) Gut Isolates. British Biotechnology Journal. 2015 5(4): 196-205.