Monitoring seasonal variation of epicatechin and gallic acid in the bark of Saraca asoca using reverse phase high performance liquid chromatography (RP‑HPLC) method.
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Date
2015-01
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Abstract
Background: Saraca asoca (Roxb.) Wilde (Fabaceae) is a high valued but vulnerable medicinal plant of Western Ghats
region. This plant is mainly known for its use in various gynecological disorders. Objective: The objective of the present
study was to investigate seasonal variation of the polyphenolic compounds viz., epicatechin and gallic acid in the bark
of S. asoca by using Reverse Phase High Performance Liquid Chromatography‑Diode Array Detector (RP‑HPLC‑DAD)
method. Materials and Methods: The bark was collected in six different Ritu (season) viz. Varsha (monsoon),
Sharad (autumn), hemant (early winter), Shishir (winter), Vasanta (spring), and Grishma (summer) mentioned in Ayurveda.
Results: The RP‑HPLC‑DAD analysis indicated that levels of epicatechin and gallic acid in the bark of S. asoca vary
seasonally. The highest concentration of epicatechin was observed in Shishir Ritu (3315.19 ± 165.76 mg/100g) and
gallic acid during Hemant Ritu (211.90 ± 10.60 mg/100 g). Conclusions: In present study, the ability to synthesize and
accumulate both the compounds in bark of S. asoca varied greatly throughout the seasons. It was also observed that the
compound epicatechin was present abundantly as compared to gallic acid throughout the seasons.
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Keywords
Ayurveda, epicatechin, gallic acid, Saraca asoca (Roxb.) Wilde, seasonal variation
Citation
Ketkar Pushkar M, Nayak Shraddha U, Pai Sandeep R, Joshi Rajesh K. Monitoring seasonal variation of epicatechin and gallic acid in the bark of Saraca asoca using reverse phase high performance liquid chromatography (RP‑HPLC) method. Journal of Ayurveda and Integrative Medicine. 2015 Jan-Mar; 6(1): 29-34.