Total polyphenolic contents and in vitro antioxidant properties of eight Sida species from Western Ghats, India.
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Date
2015-01
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Abstract
Background: Sida L., is a medicinally important genus, the species of which are widely used in traditional systems of medicine
in India. Pharmacologically, roots are known for anti‑tumor, anti‑HIV, hepatoprotective, and many other properties. Phenolic
antioxidants help in reducing oxidative stress occurring during treatment of such diseases. Objective: The study aimed to
evaluate and compare polyphenol contents and antioxidant properties of eight selected species of Sida from Western Ghats,
India. Materials and Methods: Methanolic root extracts (10% w/v) of Sida species, viz., S. acuta, S. cordata, S. cordifolia,
S. indica, S. mysorensis, S. retusa, S. rhombifolia, and S. spinosa were analyzed. Results: Sida cordifolia possessed highest
total phenolic content (TPC: 1.92 ± 0.10 mg Caffeic Acid Equivalent/g and 2.13 ± 0.11 mg Tannic Acid Equivalant/g),
total flavonoid content (TF: 2.60 ± 0.13 mg Quercetin Equivalent/g) and also possessed highest antioxidant activities in
2,2‑diphenylpicrylhydrazyl (DPPH) radical scavenging (51.31 ± 2.57% Radical Scavenging Activity, (RSA); Trolox Equivalent
Antioxidant Capacity: 566.25 ± 28.31μM; Ascorbic acid Equivalent Antioxidant Capacity: 477.80 ± 23.89 μM) and Ferric
Reducing Antioxidant Power assays (TEAC: 590.67 ± 29.53 μM; AEAC: 600.67 ± 30.03 μM). Unlike DPPH and Ferric
Reducing Antioxidant Power (FRAP) activity, 2, 2′‑Azinobis (3‑ethyl Benzo Thiazoline‑6‑Sulfonic acid) ABTS+ antioxidant
activity was highest in S. indica (TEAC: 878.44 ± 43.92 μM; AEAC 968.44 ± 48.42 μM). It was significant to note
that values of AEAC (μM) for all the antioxidant activities analyzed were higher than that of TEAC. Conclusion: The high
contents of phenolic compounds in the root extracts of selected Sida species have direct correlation with their antioxidant
properties. Conclusively, roots of S. cordifolia can be considered as the potential source of polyphenols and antioxidants.
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Keywords
Antioxidant activity, Bala, Sida, total phenolic content, total flavonoids
Citation
Subramanya M D, Pai Sandeep R, Upadhya Vinayak, Ankad Giresh M, Bhagwat Shalini S, Hegde Harsha V. Total polyphenolic contents and in vitro antioxidant properties of eight Sida species from Western Ghats, India. Journal of Ayurveda and Integrative Medicine. 2015 Jan-Mar; 6(1): 24-28.