Detection of Uropathogens by Using Chromogenic Media (Hicrome UTI agar), CLED agar and other Conventional Media.
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Date
2011-01
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Abstract
This study was undertaken to find media better than routinely used media in isolation of uropathogens.Three hundred
urine samples having pus cells >_ 5/ HPF were enrolled for the study. Comparison of isolation and identification of
uropathogens among HiCrome UTI Agar media, 5% Sheep Blood agar & MacConkey agar and CLED agar media
were done. Among them 95(31.67%) samples showed single growth, 6 (2%) showed mixed growth and 199 (66.67%)
showed no growth. Rate of presumptive identification of organisms in primary culture plate were high in HiCrome
UTI agar media. For Escherichia coli, it was 94.20% whereas in CLED agar it was 79.71% and by Blood agar and
MacConkey agar media in combination it was 82.61%. All the Enterococcus spp. were identified in HiCrome UTI
agar media, 33.33% in CLED agar media but none in Blood agar and MacConkey agar media. Among the mixed
growth, 100% organisms were identified on HiCrome UTI Agar media due to distinct colour produced by the
different organisms, whereas in one (16.67%) sample (mixed Esch.coli and Pseudomonas spp.) organisms were
identified on other three media.
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Keywords
UTI, Uropathogen, HiCrome UTI Agar media
Citation
Praveen R, Saha S K, Shamshuzzaman S M, Rashid A L, Chowdhury A, Muazzam N. Detection of Uropathogens by Using Chromogenic Media (Hicrome UTI agar), CLED agar and other Conventional Media. Faridpur Medical College Journal. 2011 Jan; 6(1): 46-50.