Effect of aged garlic extract on immune responses to experimental fibrosarcoma tumor in BALB/c mice.
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Date
2014-10
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Abstract
BACKGROUND: Aged garlic extract (AGE) has many biological activities including radical scavenging, antioxidative and immunomodulative
effects. AIM: In this research work, the antitumor and immunomodulatory effects of AGE against fibrosarcoma implanted tumor were
studied. MATERIALS AND METHODS: WEHI‑164 fibrosarcoma cells were implanted subcutaneously on day 0 into the right flank of 40 BALB/c mice
at age of 8 weeks. Mice were randomly categorized in two separate groups: First received AGE (100 mg/kg, IP), second group as the control
group received phosphate buffered saline. Treatments were carried out 3 times/week. Tumor growth was measured and morbidity was recorded.
Subpopulations of CD4+/CD8+ T cells were determined using flow cytometry. WEHI‑164 cell specific cytotoxicity of splenocytes and in vitro
production of interferon gamma (IFN‑γ) and interleukin‑4 cytokines were measured. RESULTS: The mice received AGE had significantly longer
survival time compared with the control mice. The inhibitory effect on tumor growth was seen in AGE treated mice. The CD4+/CD8+ ratio and in vitro
IFN‑γ production of splenocytes were significantly increased in AGE group. WEHI‑164 specific cytotoxicity of splenocytes from AGE mice was also
significantly increased at 25:1 E: T ratio. CONCLUSION: Administration of AGE resulted in improved immune responses against experimentally
implanted fibrosarcoma tumors in BALB/c mice. AGE showed significant effects on inhibition of tumor growth and longevity of survival times.
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Keywords
Aged garlic extract, BALB/c mice, fibrosarcoma, tumor therapy
Citation
Tabari M Abouhosseini, Ebrahimpour S. Effect of aged garlic extract on immune responses to experimental fibrosarcoma tumor in BALB/c mice. Indian Journal of Cancer. 2014 Oct-Dec; 51(4): 609-613.