Association of vascular endothelial growth factor single nucleotide polymorphisms on the prognosis of breast cancer patients.
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Date
2014-10
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Abstract
BACKGROUND: Vascular endothelial growth factor (VEGF), a major mediator of vascular permeability and angiogenesis, may play a pivotal role in
mediating the development and progression of breast cancer. In the present study, we examined the genetic variations of the VEGF gene to assess
its possible relation to breast cancer. MATERIALS AND METHODS: A total of 200 patients with histologically confirmed cases of breast cancer and 200
healthy women were genotyped for VEGF single nucleotide polymorphisms (405G > C and −1154G > A) by polymerase chain reaction‑restriction
fragment length polymorphism analysis. Pre‑operative plasma VEGF levels were determined by enzyme‑linked immunosorbent assay in 200 women
with breast cancer and in 200 normal female controls. RESULTS: The genotype frequencies of the +405G > C, −1154G > A polymorphisms did not
show a significant deviation from the Hardy‑Weinberg expectation. The minor allele frequencies of the +405G > C and −1154G > A polymorphisms
among cases and controls were 33.5% (C allele), 31.5% (A allele) and 35% (C allele), 34.5% (A allele) respectively. +405GG and −1154GG
genotypes were associated with higher levels of VEGF among breast cancer cases and controls. Increased plasma VEGF levels were significantly
associated with, clinical stage of the disease (P = 0.035). CONCLUSION: Although none of the polymorphisms were significantly associated with
breast cancer, some of the VEGF genotypes may influence tumor growth through an altered expression of VEGF and tumor angiogenesis.
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Keywords
Breast cancer, plasma vascular endothelial growth factor, vascular endothelial growth factor polymorphism
Citation
Rani J, Rahul B, Ramesh G, Krishnamoorthy L, Shilpa C, Ramaswamy G, Deshmane V. Association of vascular endothelial growth factor single nucleotide polymorphisms on the prognosis of breast cancer patients. Indian Journal of Cancer. 2014 Oct-Dec; 51(4): 512-517.