Genotypic Detection of Epstein-Barr Virus in Pediatric Transplant Recipients From India.
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Date
2015-11
Authors
Janani, Madhuravasal Krishnan
Malathi, Jambulingam
Rela, Mohamed
Farouk, Mohammed
J, Padmapriya
Madhavan, Hajib N
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
Objective: To determine the rate of occurrence and genotypes of
Epstein-Barr Virus (EBV) among pediatric renal and liver
transplants recipients.
Design: Observational study.
Setting: Vision Research Foundation referral center and Institute
of Liver Disease and Transplantation, Chennai, India.
Participants: 70 pediatric solid organ transplant recipients and 60
voluntary healthy donors.
Methods: Polymerase chain reaction (PCR) for detection and
genotyping of EBV were carried out using genes targeting Viral
capsid antigen, Nuclear antigen 1, 2 and 3, followed by real time
PCR for viral load determination and further confirmed by
phylogenetic analysis.
Results: EBV was detected in 35 (51.4%) samples (32 liver and 4
renal transplants) with high viral load. Type A was detected in 33
samples, Type B in 2 liver transplant patients, and co-infection in
one liver transplant patient who developed Post-transplant
Lymphoproliferative Disorder (PTLD). Real time PCR results
correlated with conventional PCR. The mean viral load for
patients who did not develop PTLD was 50,424 copies/mL.
Overall EBV load in patient with PTLD ranged from 1,40,392
copies/mL prior to PTLD diagnosis to 62,124 copies /mL post
treatment.
Conclusion: EBV infection is the high risk factor for PTLD after
liver transplantation. PCR targeting of EBV can be applied to
diagnose EBV infections and monitor treatment for EBV in
pediatric solid organ transplant recipients.
Description
Keywords
Phylogenetic analysis, Polymerase chain reaction, Post-transplant lymphoproliferative disorder
Citation
Janani Madhuravasal Krishnan, Malathi Jambulingam, Rela Mohamed, Farouk Mohammed, J Padmapriya, Madhavan Hajib N. Genotypic Detection of Epstein-Barr Virus in Pediatric Transplant Recipients From India. Indian Pediatrics. 2015 Nov; 52(11): 946-950.