Comparision of the conventional diagnostic techniques,BACTEC and PCR.
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Date
2007-10
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Abstract
Two hundred and thirty samples from suspected pulmonary and extra pulmonary cases of tuberculosis
were processed for detection of Mycobacterium tuberculosis by ZN smear examination, LJ medium
culture, BACTEC radiometric culture and Polymerase Chain Reaction tests. A significant difference was
seen in the sensitivities of different tests,ie.73.9% for PCR tests,34.78% for ZN smear examination,
52.17% for LJ culture and 58.69% for BACTEC culture. However,there was no significant difference in
specificity of different tests(P> 0.05). PCR test sensitivity in pulmonary and extrapulmonary clinical
samples was 74.0% and 78.5% respectively and found to be significantly higher (P<0.05) when compared
with those of other tests. The mean detection time for M.tuberculosis was 24.03 days by LJ medium
culture, 12.89 days by BACTEC culture and less than one day by PCR test.
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Keywords
Tuberculosis, M.tuberculosis, PCR, LJ medium, BACTEC
Citation
Oberoi Aroma, Aggarwal Aruna. Comparision of the conventional diagnostic techniques,BACTEC and PCR. JK Science Journal of Medical Education and Research. 2007 Oct-Dec;9(4): 179-181