Fungal Profile and Susceptibility Pattern in Cases of Keratomycosis
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Date
2006-01
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Abstract
The present study was undertaken to find out various fungi causing keratomycosis and to determine the
antifungal susceptibility profile of these isolates. One hundred corneal scrapings from patients with corneal
ulcers were subjected to KOH wet mount preparation. Thirty samples which were positive for fungal
elements on direct microscopic examination were cultured on Sabouraud's dextrose agar medium(SDA).The
isolates were identified and were subjected to drug susceptibility tests for ketoconazole and fluconazole by
tube dilution method. Incidence of fungal corneal ulcers was maximum in the age group of 20-49 years
(56.67%). Agriculturists constituted the largest group (53.33%), history of trauma was present in 90% of
the patients. Aspergillus species were the commonest isolates (70%) followed by candida (20%), alternaria
(6.70%) and penicillium (3.30%). MIC of ketoconazole varied from 0.5mg/ml to 10 mg/ml. MIC of
fluconazole varied from 0.5 - 10 mg/ml and 19 isolates of fungi did not show any sensitivity to fluconazole
upto concentration of 10 mg/ml which was the upper limit of the test system. As agricultural activity and
related ocular trauma were principal causes of mycotic keratitis, KOH wet mount preparation is an
essential tool in the diagnosis of these infections. It is a very simple and sensitive method. Susceptibility
pattern to antifungal drugs was determined to optimize therapeutic response in eye infections.
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Keywords
Keratomycosis, Aspergillus, Minimum Inhibitory Concentration (MIC), Antifungal Agents
Citation
Arora Usha, Aggarwal Aruna, Joshi Vijay. Fungal Profile and Susceptibility Pattern in Cases of Keratomycosis. JK Science Journal of Medical Education and Research. 2006 Jan-Mar;8(1): 39-41