Volume, conductivity, and scatter parameters as diagnostic aid to bacterial sepsis: A tertiary care experience.
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Date
2015-10
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Abstract
Introduction and Materials and Methods: Early diagnosis of sepsis is extremely
important to reduce high mortality and morbidity. In this study, clinical usefulness
of the volume, conductivity and scatter parameters (mean channels of cell
volume, conductivity, and light scatter) in neutrophils was analyzed for predicting
acute bacterial infection, which are obtained by the Coulter LH 750 Hematology
Analyzer (Beckman Coulter, Fullerton, CA, USA) during automated differential
counts. Results: Peripheral blood samples from 162 patients with positive
blood cultures for bacteria and 40 healthy controls were studied. We observed a
significant increase in the mean channel of neutrophil volume (MNV) from septic
patients compared with control subjects (156 ± 13.5 vs. 143 ± 4.8; P < 001).
Discussion and Conclusion: An elevation of the MNV was associated with a
higher white blood cell count and percentage of neutrophils and was present
even in patients who did not have leukocytosis or neutrophilia. With a cut-off of
149 for the MNV, a specificity of 91.4% and sensitivity of 88.7% were achieved.
As a quantitative, objective, and more sensitive parameter, we propose that the
MNV has a potential to be an additional indicator for acute bacterial infection.
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Keywords
Neutrophilia, leukocytosis, sepsis, volume, conductivity and scatter parameters
Citation
Purohit Abhishek H L, Kumar Praveen, Sharma Subhadra, Kapil Arti, Gupta Ayush, Mukhopadhyay Ashok K. Volume, conductivity, and scatter parameters as diagnostic aid to bacterial sepsis: A tertiary care experience. Indian Journal of Pathology & Microbiology. 2015 Oct-Dec 58(4): 459-463.