Antimicrobial and antifungal efficacy of Spilanthes acmella as an intracanal medicament in comparison to calcium hydroxide: An in vitro study.
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Date
2015-09
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Abstract
Background: Repeated endodontic failures are due to pathogens like Enterococcus faecalis and
Candida albicans which are resistant to the common intracanal medicaments like calcium
hydroxide (Ca[OH]2).
Aims: To determine the role of commonly used folk medicine, Spilanthes acmella (SPA) against
root canal pathogens like E. faecalis, C. albicans, Staphylococcus aureus, Streptococcus sp, and
to compare its efficacy with Ca(OH)2, a popularly used intracanal medicament.
Materials and Methods: Bacterial strains of S. aureus, Streptococcus sp., E. faecalis, and fungal
strains of C.albicans were tested against different concentrations of SPA and Ca(OH)2. Seven
days old cultures of test organisms were seeded onto agar plates and uniformly spread with a
spreader. Five to six wells (8 mm) were made on agar plate to which different concentrations
of the test solutions were added. The inoculated plates were kept in an incubator at 37°C for
48 h and inhibition zones were measured.
Statistical Analysis: Results of SPA were compared with results of Ca(OH)2 statistically using
Mann–Whitney U test.
Results: A significant zone of inhibition was obtained with SPA and was found to increase as
the concentration increased for C. albicans. SPA showed a significant zone of inhibition at 2–5%
and 10% whereas Ca(OH)2 showed a zone of inhibition only at 10% for E. faecalis. SPA showed
a zone of inhibition only at 10%, whereas, Ca(OH)2 showed a significant zone of inhibition at
5% and 10% for S. aureus and Streptococcus sp., respectively.
Conclusion: SPA possesses remarkable antibacterial and antifungal activity against common
root canal pathogens which are responsible for repeated endodontic failures such as E. faecalis
and C. albicans when compared with medicaments like Ca(OH)2.
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Keywords
Calcium hydroxide, intracanal medicament, Spilanthes acmella, zone of inhibition
Citation
Sathyaprasad Savitha, Jose Bijosh K, Chandra H Sharath. Antimicrobial and antifungal efficacy of Spilanthes acmella as an intracanal medicament in comparison to calcium hydroxide: An in vitro study. Indian Journal of Dental Research. 2015 Sept-Oct; 26(5): 528-532.