Quantitative evaluation of apical extrusion of intracanal bacteria using rotary ProTaper, K3XF, twisted and hand K‑file system: An ex vivo study.
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Date
2015-07
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Abstract
Aims: The aim of this study was to evaluate the number of intracanal bacteria extruded apically
during root canal preparation using rotary ProTaper, K3XF, twisted, and hand K‑file system.
Subjects and Methods: Seventy extracted single‑rooted human mandibular premolar teeth were
used. Access cavities were prepared and the teeth were mounted in glass vials. Root canals were
then contaminated with a pure culture of Enterococcus faecalis (ATCC 29212) and incubated at
37°C for 24 h. The contaminated roots were divided into four experimental groups of 15 teeth
each and one control group of 10 teeth. Group 1: ProTaper; Group 2: K3XF; Group 3: Twisted
file; Group 4: Hand K‑file; Group 5: Control group. Bacteria extruded from the apical foramen
during instrumentation were collected into vials. The microbiological samples were incubated
in culture media for 24 h. Colonies of bacteria were counted and the results were given as
number of colony‑forming units (CFU)/ml.
Statistical Analysis Used: The obtained data were analyzed using the Kruskal–Wallis one‑way
analysis of variance and Mann–Whitney U‑tests.
Results: There was a significant difference between the rotary and hand instrumentation system
related to the apically extruded intracanal bacteria.
Conclusions: Both the rotary and hand instrumentation systems extruded intracanal bacteria
through the apical foramen. K3XF file system showed least bacterial extrusion amongst all
instrumentation groups.
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Keywords
Enterococcus faecalis, K3XF, K‑file, ProTaper, twisted
Citation
Ghogre Priyanka, Chourasia Hemant Ramesh, Agarwal Manish, Singh M P, Gurav Sandeep, Ghogre Rahul. Quantitative evaluation of apical extrusion of intracanal bacteria using rotary ProTaper, K3XF, twisted and hand K‑file system: An ex vivo study. Indian Journal of Dental Research. 2015 July-Aug; 26(4): 406-410.