Partial Characterization of Replicase Gene of Tobacco Streak Ilarvirus in Onion (Allium Cepa.L).
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Date
2015-07
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Abstract
Tobacco streak virus (TSV) is an important emerging virus belongs to the genus Ilarvirus and
family Bromovidae. The Onion crop is infected by Tobacco streak ilar virus and it is major problem in different
places of Andhra Pradesh in South India and also transmitted by thrips vector. TSV suspecting onion samples were
identified by direct antigen coating enzyme linked immunosorbent assay using TSV polyclonal antiserum. The
Replicase gene from each isolate was amplified using TSV replicase gene specific primers by using the RT-PCR.
The ~530 bp product was amplified, cloned, sequenced and determined its length as 534 nucleotides and codes for
178 amino acids. The partial sequence of TSV-Rep shared identity of 87.6 -99.8% at nucleotide levels and 67.8-
99.4% at amino acid levels respectively with other reported TSV isolates. The phylogenetic tree relationship based
on the nucleotide sequence of present study isolate (AP-Onion-Chittoor) from different geographical regions was
also analyzed in this study.
Description
Keywords
DAC-ELISA, Onion, RT-PCR, Tobacco streak virus
Citation
Sujitha A, Reddy B V Bhaskara, Sivaprasad Y, Usha R. Partial Characterization of Replicase Gene of Tobacco Streak Ilarvirus in Onion (Allium Cepa.L). International Journal of Applied Biology and Pharmaceutical Technology. 2015 Jul-Sept; 6(3): 155-161.