Improved RP-HPLC Method for Quantitation of Swertiamarin from Swertia Densifolia Leaves and Marketed Herbal Formulations.
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Date
2015-07
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Abstract
Objective: Present study aims at developing and validating an improved RP-HPLC method for determination of
Swertiamarin from extracts of Swertia densifolia (Griseb.) Kashyap leaves and marketed herbal formulations.
Methods: Developed RP-HPLC method is validated and is used for determination of Swertiamarin from hydroalcoholic
extracts of test plant material and marketed herbal formulations. The separation is carried out on C18 column. The mobile
phase used is 10 mM Ammonium acetate: Acetonitrile: Formic acid in ratio 90:10:0.2% (v/v/v/). The wavelength selected
is 237 nm using UV detector.
Results and Discussion: A seven-point calibration curve over the concentration range of 50.00 –20000.00 ng/mL for
Swertiamarin provides an optimum linear detector response (with r2>0.9970). The % R.S.D. (n = 6) for the low, middle
and high quality control samples are within <6.44 and < 9.04 for inter-day and intra-day precision and accuracy
respectively.
Conclusion: A simple, sensitive and precise method is developed for quantitation of SWM. Method is validated as per
International Conference on Harmonization (ICH) guidelines and its applicability is demonstrated by successful
measurement of Swertiamarin from hydroalcoholic extracts of Swertia densifolia leaves and selected marketed herbal
formulations.
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Keywords
RP-HPLC, Swertiamarin, Swertia densifolia (Griseb.) Kashyap, Analytical Method Validation, International Conference on Harmonization (ICH)
Citation
Gandhi Kanchan, Menon Sasikumar, Shailajan Sunita. Improved RP-HPLC Method for Quantitation of Swertiamarin from Swertia Densifolia Leaves and Marketed Herbal Formulations. International Journal of Applied Biology and Pharmaceutical Technology. 2015 Jul-Sept; 6(3): 24-31.