Antiangiogenic and Proapoptotic Activity of Averrhoa Carambola L. Fruit Extract on Ehrlich Ascites Carcinoma Treated Mice.
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Date
2015-04
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Abstract
Aim: Accumulation of fluid containing cancer cells in the abdomen particularly, in ovarian cancer, forms
malignant ascites rendering poor prognosis at this stage. We investigated the tumor inhibitory activity of
Averrhoa carambola L. fruit extract on EAC cells administered mice targeting angiogenesis and apoptosis, and the
bioactive compounds responsible.
Main Methods: Body weight, ascites volume and peritoneal angiogenesis were monitored. Giemsa staining on EAC
cells, DNA fragmentation assay and FACS analysis to determine the growth arrest were conducted. VEGF count was
monitored using ELISA. Phytochemical screening and HPLC analysis were conducted to determine the bioactive
compounds.
Key Findings: The fruit extract expressed direct cytotoxicity to EAC cells by inducing apoptosis as evidenced
by decrease in tumor volume, viable cell count and body weight of EAC bearing mice; characteristic
apoptotical features, DNA fragmentation of apoptosis, and growth arrest taking place at G2/M phase of the
cell cycle. Significant decrease in density of microvessel network in peritoneal lining and VEGF count in
treated cells indicated that the fruit extract curbed malignancy of tumor through its antiangiogenic activity
also. All these can be attributed to catechin, epicatechin and ferulic acid present in the extract. The total phenolic,
flavanoids, proancthocyanidin and condensed tannins content were 1.216 mgGAE/g extract, 767 mgCE/g extract,
586 mgCE/ g extract and 18.35 mgCE/g extract respectively.
Significance: The present study is the first to provide direct evidence that Averrhoa carambola L. has potent
proapoptotic and antiangiogenic activity which may contribute to its well- documented clinical activity as a
pharmaceutical drug.
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Keywords
Angiogenesis, Ehrlich Ascites Carcinoma cells, FACS, apoptosis, VEGF
Citation
Chauhan Jyoti Bala, Kapfo Wethroe, Jayarama Shankar, Balaji Kyathegowdanadoddi S. Antiangiogenic and Proapoptotic Activity of Averrhoa Carambola L. Fruit Extract on Ehrlich Ascites Carcinoma Treated Mice. International Journal of Applied Biology and Pharmaceutical Technology. 2015 Apr-June; 6(2): 246-254.