The Effect of Media and Light on In Vitro Sporulation of Alternaria Solani.
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Date
2015-01
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Abstract
Early blight of tomato incited by Alternaria solani is an economically significant disease especially in
commercial tomato cultivation under greenhouse and field conditions. Since, A. solani is a shy sporulator, the present
investigation was taken to assess the optimum in vitro conditons for growth and sporulation of early blight pathogen.
Ten A. solani isolates obtained from diseased leaf samples collected different crop growing areas of India were used in
the present study. The effect of different incubation periods, fluorescent light, cold-water treatment and media were
evaluated. Our results revealed maximum sporulation of A. solani on tomato fruit extract agar medium (TFEA) under
continuous light for 7 days at 250C, followed by cold-water treatment and further incubated in darkness at 200C for 48 h.
The sporulation of test pathogen was however sparse on V-8 juice agar. Further, the A. solani isolates on V-8 juice agar
did not respond to the treatments imposed. Among the isolates, JAS (Jhajjar) isolate, that is more versatile in its ability
to produce spores recorded irregular margin with abundant aerial mycelium.
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Keywords
Alternaria solani, early blight of tomato, sporulation, fluorescent light, tomato fruit extract agar
Citation
Varma P Kishore, Yamuna C, Mangala U N. The Effect of Media and Light on In Vitro Sporulation of Alternaria Solani. International Journal of Applied Biology and Pharmaceutical Technology. 2015 Jan-Mar; 6(1): 97-102.