Development of RP-HPLC method for the estimation of Rasagiline mesylate in bulk and tablet dosage forms.
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Date
2012
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Abstract
A simple RP-HPLC method for the determination of Rasagiline Mesylate in bulk and tablet dosage form was devel-oped. Numerous HPLC conditions were tested for determination of rasagiline. The best result was achieved by using Purosphere star RP-18, (150×4.6mm), 5μm column and a mobile phase consisting of Potassium Orthophosphate: Acetonitrile (60:40 v/v) adjusted to pH 7.0(±0.05) with Ammonia solution, a flow rate of 1.5 ml/min with ultraviolet detection at 210nm. The correlation coefficients for calibration curves within the detection range of 5-30μg/ml were 0.9993. The within and between-day precision was determined for both retention time and peak area. The retention time of rasagiline is 6.0 minutes.
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Keywords
Rasagiline, Purosphere star RP-18, Tablets, Estimation, RP-HPLC, Validation, Method development
Citation
Raj Napa Delhi, Rao Abburu Rukmangada, Bhimanadhuni Chusena Narasimharaju. Development of RP-HPLC method for the estimation of Rasagiline mesylate in bulk and tablet dosage forms. International Current Pharmaceutical Journal. 2012; 1(9): 285-287.