The Influence of Epithelial Sodium Channels in Isolated Goat Ileum Segment; Interaction with Steroids.
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Date
2014-01
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Abstract
The contractile effect of Acetylcholine (Ach) in the isolated longitudinal ileal muscle of adult goats
was studied over a varying concentration range. Ach produced a concentration dependent-response curve indicative
of an interaction with muscarinic receptors in the ileum, with a maximum contraction seen at 12 μM. On the other
hand, pretreatment with the ENaC blocker, Amiloride (100 μM) substantially reduced the Ach induced contractions
by 67.11 %. However, pretreatment with Prednisolone (2mM) restored this effect and the relaxation induced was
only 14.26 %. This change was found to be statistically significant. This study emphasizes the importance of ENaC
channels in the goat intestinal smooth muscle.
Description
Keywords
Epithelial sodium channel, Amiloride, Prednisolone, Ileum, Acetylcholine
Citation
Varkey Dhaya R, Mathew Sumith K, Tyagi Manoj G. The Influence of Epithelial Sodium Channels in Isolated Goat Ileum Segment; Interaction with Steroids. International Journal of Applied Biology and Pharmaceutical Technology. 2014 Jan-Mar; 5(1): 82-84.