Purification of Catalase Enzyme from Pleurotus Ostreatus.
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Date
2014-01
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Abstract
The oyster mushroom Pleurotus ostreatus is the most commonly cultivated mushroom, and are effective
for antitumor, antibacterial, anti viral and hematological agents and in immune modulating treatments. Several
compounds from oyster mushrooms, potentially beneficial for human health have been isolated and studied. The aim of
this research is to purify an enzyme catalase from Pleurotus ostreatus through Sephadox G-75 column, its molecular
weight was determined by polyacrylamide gel electrophoresis and the catalase enzyme stability were observed at
various temperature and different pH condition. Under denaturing conditions, polyacrylamide gel electrophoresis
revealed dissociation of a major component of molecular weight 62,000 kDa, which constituted 90% of the total
protein of the stained gel, suggesting that the native enzyme is tetrameric. The optimum temperature and pH for the
purified enzyme catalase from Pleurotus ostreatus enzymatic reaction were 30°C and pH 7.5.
Description
Keywords
Pleurotus ostreatus, Catalase, polyacrylamide gel electrophoresis
Citation
Susmitha S, Vidyamol K K, Ranganayaki P, Vijayaraghavan R. Purification of Catalase Enzyme from Pleurotus Ostreatus. International Journal of Applied Biology and Pharmaceutical Technology. 2014 Jan-Mar; 5(1): 28-34.