In-Vitro Micropropagation and Media Optimization of Stevia Reboudiana an Important Bio-Sweetener and Medicinal Plant”.
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Date
2014-01
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Abstract
The technique of Plant Biotechnology has an important role to play in the production of agriculture,
horticulture and ornamental plants and in the manipulation of plants for improved agronomic performance. In the
present study initiation of callus has been done by using explants from auxiliary and apical meristems of Stevia
rebaudiana. Explants were inoculated on MS basal medium having Vitamin supplement with Auxin like 2-4, D and
NAA (0.5mg/lt. -2.0mg/lt.) alone or in combination with cytokinis like BAP & Kinetin (0.52 mg/lt. -1.0 mg/lt.).
Sucrose 30 gm. and agar 4.5 gm./lt. After the formation of explants, they were transferred into shoot induction
medium containing different concentration of cytokine like BAP & Kinetin (0.5 mg/lt. to 30 mg/lt.) with additional
vitamins. After few days numbers of multiple shoots were formed. Strong and elongated shoots were treated with root
initiating medium i.e MS containing Auxin like IBA, NAA & activated charcoal. Maximum 60-70% callusing in 10 to
15 days was initiated on 2-4D (1-2mg/lt.) and 40-50% callusing were reported in medium containing (1.0 NAA & 0.5
mg/lt. BAP) in 25-30 days. Nodular compact cells were formed in 2-4D. The medium containing BAP alone showed
shoot formation 70-80% with 40% coconut water. Second rooting medium used was containing NAA and activated
charcoal and 85% rooting were observed within 7-10 days after transferring. Finally the cultured plants were
transferred for hardening.
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Keywords
MS Media, Stevia rebaudiana, Auxin, cytokine, Kinetin
Citation
Pasha Farhina. In-Vitro Micropropagation and Media Optimization of Stevia Reboudiana an Important Bio-Sweetener and Medicinal Plant”. International Journal of Applied Biology and Pharmaceutical Technology. 2014 Jan-Mar; 5(1): 12-16.