Genetic Divesity and Molecular Characterization of Mungbean Genotypes (Vigna radiata (L.) Wilczek).
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Date
2013-10
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Abstract
The present investigation was undertaken to examine the genetic divergence in 50 mungbean
germplasm lines for 13 characters using Mahalanobis D2 statistics. The genotypes grouped into eight clusters. Cluster
VII had maximum intra-cluster distance while inter-cluster distance was highest between clusters V and VII. Cluster
means indicated that none of the clusters was superior for all the characters studied. Therefore, hybridization between
genotypes belonging to different clusters is suggested for development of superior genotypes. 10 SSR primers were
used for molecular study of which only one gave slight difference among 19 mungbean genotypes. The quality and
quantity of DNA used for amplification by PCR is the key to reproducible results and success of genotyping.
Especially, DNA purity is extremely crucial for obtaining clear and discriminate patterns. DNA extraction from
mungbean is difficult due to presence of contaminants such as phenols. Therefore, the present study was under taken
to obtain high quality and pure DNA in mungbean. With few modifications four different DNA extraction protocols
were tried in the present study to obtain high quality and pure DNA viz., (I) Doyle and Doyle (1987), (ii) Method of
Murray and Thompson (1980), (iii) Porebski et al.(1997), and (iv) Lin et al. (2001). Out of the four methods tried
for DNA extraction, the method of Lin et al. (2001) was found most efficient, as the DNA obtained through this
protocol was relatively pure which gave amplyfying products in the PCR. The genotype used for the standardization
was MGG -361. Molecular characterization of 19 randomly chosen mungbean genotypes was attempted with the eight
standardized primers. None of the primers showed scorable polymorphism. The primers VR4, VR5 and VR9, exhibited
non specific bands, in addition to the monomorphic bands.
Description
Keywords
Mungbean, D2 statistics, Genetic diversity, DNA extraction, Standardization, SSR primers
Citation
Prasanna B Laxmi, Rao P J M, Murthy K G K, Prakash K Kiran, Yamini K N, Srividhya A. Genetic Divesity and Molecular Characterization of Mungbean Genotypes (Vigna radiata (L.) Wilczek). International Journal of Applied Biology and Pharmaceutical Technology. 2013 Oct-Dec; 4(4): 151-160.