Evaluation of anti-tumor activity of Momordica cymbalaria fenzl.
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Date
2015-07
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Abstract
Background: The aim of the current study is to evaluate the anti-tumor activity
of saponins isolated from the roots of Momordica cymbalaria (MC) against
dimethylbenz[a]anthracene (DMBA) induced rats.
Methods: A steroidal saponin MC (SMC) was isolated from MC fenzl and purified
by preparative high-performance liquid chromatography. Breast cancer was induced in
50-day-old female Sprague-Dawley rats by injecting DMBA (6 mg/kg intravenous) in
three doses on day 50, 54, and 57. The rats were randomized into four groups; control,
DMBA, SMC (100 mg/kg), and tamoxifen (6.6 mg/kg) to DMBA breast cancer rats. The
tumor size, volume, hormonal, antioxidant, and whole mount parameters were estimated.
Results: Mean tumor size and volume, luteinizing hormone, and progesterone
with superoxide dismutases, catalase, and glutathione levels increased significantly
(p<0.001); serum estradiol, follicle stimulating hormone with lipid peroxidation
decreased significantly (p<0.001) in DMBA-induced breast cancer and vice versa in
SMC and tamoxifen. Terminal end buds, terminal ducts, alveolar buds, and lobules
decreased significantly (p<0.001) in DMBA-induced breast cancer whereas increased
significantly in SMC and tamoxifen. Histological necrosis and hemorrhage along
with focal desmoplastic reaction in DMBA-induced breast cancer; ductile elongation
and hyperplasia of both ducts and alveoli were prominent, with increased secretory
activity in SMC group. The results confirmed the chemopreventive effect of SMC
and tamoxifen in DMBA-induced breast cancer.
Conclusions: The SMC exhibited anti-tumor activity against mammary cancer,
which may be due to its anti-estrogenic, antioxidant activity.
Description
Keywords
Antiestrogen, Antioxidant, Breast tissue, Dimethylbenz[a]anthracene, Hormonal parameters, Mammary cancer, Momordica cymbalaria, Saponins, Whole mount parameter
Citation
Kaskurthy Rupa Lavanya, Koneri Raju Balawanth, Samaddar Suman. Evaluation of anti-tumor activity of Momordica cymbalaria fenzl. International Journal of Basic & Clinical Pharmacology. 2015 Jul-Aug; 4(4): 779-786.