Beneficial effects of Morus alba and Azadirachta indica leaf extract based combination cream in scalding type burn injury in rats.
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Date
2015-07
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Abstract
Background: The present study was designed to evaluate the potential of a
combination cream of aqueous extracts of leaves of Morus alba (MA) and Azadirachta
indica (AI) in scalding type burn wound injury in rats.
Methods: Plant material was successively extracted and aqueous extracts were
selected. Three extract based cream formulations viz. 20% w/w (MA), 20% w/w
(AI), and combination cream containing 10%+10% w/w (MA+AI) were prepared.
Cream base and standard anti-burn cream containing silver sulfadiazine were also
used for comparison. Scalding type burn was given by pouring water at 90°C on a
shaved dorsal area of 20 mm2. Deep second-degree burn injury was produced which
was evaluated for next 21 days for a percentage of wound contraction and period of
epithelialization. On 21st day, animals were sacrificed and histopathological slides
were prepared using hematoxylin-eosin staining. Burned tissue was also screened
for levels of oxidative stress using thiobarbituric acid reactive substances (TBARS)
and glutathione (GSH) estimation.
Results: There was a significant increase in the percentage of wound contraction and
significant decrease in period of epithelialization in MA, AI, and MA+AI combination
cream treated group as compared with control group. However, most significant
results were obtained with MA+AI combination cream. Histologically, MA+AI
cream treatment resulted in almost complete re-epithelialization and restructuring
of the wound tissue. There was a significant rise in TBARS and decrease in GSH
levels in burn injury group which was reversed to a major extent by the application
of combination cream.
Conclusions: The results indicate toward the possible role of free radical scavenging
potential of extracts in the Burn wound tissue healing.
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Keywords
Morus alba, Azadirachta indica, Scalding, Cream, Burn injury, Free radicals
Citation
Bhatia Nitish, Sharma Raghuveerv, Kaur Gursharan, Kaur Narinder, Singh Gurwinder, Dhawan Ravi. Beneficial effects of Morus alba and Azadirachta indica leaf extract based combination cream in scalding type burn injury in rats. International Journal of Basic & Clinical Pharmacology. 2015 Jul-Aug; 4(4): 720-728.