Functional nitric oxide conjugate systems state/restored heart thiols of rats in modeling isadrine-pituitrin’s myocardial infarction using metabolite-tropic cardioprotector “Angiolin.
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Date
2015-01
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Abstract
Background: According to modern researches, endothelial dysfunction (ED) is one of
the primary pathogenetic elements of cardiovascular diseases (myocardial infarction
[MI], ischemic heart diseases, cerebral ischemic stroke, atherosclerosis, arterial
hypertension, pulmonary hypertension, heart failure, and dilated cardiomyopathy)
as well as obesity, hyperlipidemia, diabetes and hyperhomocysteinemia. The aim of
this work was to study the infl uence of potential metabolitotropic cardioprotector
“Angiolin” on the parameters of conjugate systems nitric oxide (NO)/restored thiols
in heart under isadrine-pituitrin MI.
Methods: This study was performed on Wistar white rats weighing 190-210 g.
Biochemical, immune-enzyme analysis and histoimmunechemical study were
performed.
Results: In histological sections of hearts of the rats receiving Angiolin in parenteral
dosing 50 mg/kg 30 mins before each pituitrin injection the density of endothelial NOsynthase
(NOS)-positive cells increased by 29% and the density of inducible NOSpositive
cells decreased by 23.3%. In cytosolic fraction of myocardium homogenate
NOS activity increased by 27%, the concentration of NO stable metabolites increased
by 70% and the content of nitrosative stress marker nitrotyrosine decreased by 42%
when compared with control group. At the same time in similar samples of heart
homogenate the increase of restored thiol groups’ level by 53.3%, methionine - by
35.1%, cysteine - by 170% and activity of glutathione reductase - by 186% was
noted. The administration of reference drug mildronate to the animals with MI in
dose 100 mg/kg did not result in signifi cant changes of the studied parameters of
thiol-disulfi de system and NO system of the heart when compared with control group.
Conclusions: Angiolin does not infl uence directly on NOS in MI, but at the same
time protects NO from nitrosative stress increasing restored equivalents of thioldisulfi
de system.
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Keywords
Metabolitotropic cardioprotector, Angiolin, Myocardial infarction
Citation
Belenichev Igor F, Kucherenko Lyudmila I, Nagornaya Elena A, Mazur Ivan A, Bidnenko Alexander S, Bukhtiyarova Nina V, Avramenko Nikolay A. Functional nitric oxide conjugate systems state/restored heart thiols of rats in modeling isadrine-pituitrin’s myocardial infarction using metabolite-tropic cardioprotector “Angiolin. International Journal of Basic & Clinical Pharmacology. 2015 Jan-Feb; 4(1): 15-19.